Protein A ELISA kit (Flexible Protocol)

Product Specifications

Certification

RUO

Species Reactivity

Species independent

Applications

Colorimetric detection, ELISA

Wavelength

450 nm

Sensitivity

9.01 pg/ml (range 15.63 – 1,000 pg/ml)

Compatibility

This product is compatible with the Absorbance 96 Plate Reader .

Shipping Conditions

Blue Ice

Storage Conditions

+4°C

Notes

Assay recognition of different Protein A constructs from natural Protein A from S. aureus (M), recombinant Protein A from E. coli (R), recombinant Cys-Protein A from E. coli (G), and recombinant alkaline-resistant Protein A variant from E. coli (S) were tested and the resulting concentrations were interpolated from kit standard curve and displayed in the figure provided. Application Notes: Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics

Applications Notes

For the quantitative determination of residual protein A contamination in protein A purified IgG preparations from any species.

Microtiter plate, Conjugate, Antibody, Dissociation buffer, Neutralization buffer, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2