Proteasome 20S β2 subunit monoclonal antibody (MCP165)

Product Specifications

Product Name Alternative

Proteasome subunit β type-7, Macropain chain Z

Certification

RUO

Gene ID

PSMB7 (gene name)

UniProt

Q99436 (human), P70195 (mouse)

Host

Mouse

Species Reactivity

Human,Mouse

Immunogen

Dinitrophenylated proteasomes.

Clone

MCP165

Source

Purified from hybridoma tissue culture supernatant.

Applications

IHC, WB

Purification

Protein G affinity purified.

Stability

Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.

Dilution

Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.

Specificity

Recognizes the β2 subunit of the 20S proteasome.

Formulation

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Applications Notes

Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations. Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue. Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.