Proteasome 20S α6 subunit monoclonal antibody (MCP20)
Product Specifications
Product Name Alternative
PROS30, Proteasome subunit α type-1, Macropain subunit C2, Proteasome ν chain
Certification
RUO
Gene ID
PSMA1 (gene name)
UniProt
P25786
Host
Mouse
Species Reactivity
Human,Rabbit
Immunogen
Human placenta derived proteasomes.
Clone
MCP20
Source
Purified from ascites.
Applications
IHC, IP, WB
Purification
Protein G affinity purified.
Stability
Aliquot undiluted antibody into smaller volumes (not less than 10μL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
Specificity
Recognizes the α6 subunit of proteasome 20S.
Formulation
Liquid. In PBS containing 10mM sodium azide.
Applications Notes
Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE. Western Blot: Single dimension SDS-PAGE of a human placental proteasome preparation or HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of approximately 33kDa.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
Cross Reactivity Details
Does not cross-react with rat or mouse protein.
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