Proteasome 20S core subunits polyclonal antibody

Western blot – The antibody has been characterised by single dimension SDS-PAGE using both purified human and yeast 20S proteasome and a number of tissue/cell preparations including a human placental proteasome preparation, a HeLa cell lysate, a RSV 3T3 mouse fibroblast cell lysate, and a yeast whole cell extract. Western blotting shows bands attributable to ‘core’ subunits at ~25-30kDa as shown opposite. Differences in subunit band intensity are noted between the samples. 2-D SDS-PAGE followed by Western blotting of purified 20S proteasome shows the presence of five/six spots with the following proteasome subunit correlation: α5/α7, β1, β5, β5i, β7. The 2-D patterns observed with human and mouse derived materials are not identical with some differences being observed in the molecular weights and isoelectric points of some subunits.