Proteasome 19S Rpt5/S6a subunit monoclonal antibody (TBP1-19)

The hybridoma secreting the antibody to subunit Rpt5/S6a was generated by fusion of splenocytes from Balb/c mice that had received repeated immunisation with a full length human recombinant protein with Sp2/0-Ag14 myeloma cells. The antibody (clone TBP1-19) has been characterised by one-dimensional Western blotting. Immunoblotting – Single dimension SDS-PAGE of a purified rabbit 26S proteasome preparation followed by Western blotting at optimal dilution (1:2500) gives a major band with a relative molecular weight of approximately 50kDa. There is a minor band observed running slightly slower that may be due to modification. Immunoprecipitation – This antibody has not been characterised for this purpose. Immunohistochemistry – This antibody has been shown to be suitable for this purpose in the subcellular localisation of proteasomes.