Procathepsin V (human), (recombinant) (His-tag)
Product Specifications
Specifications
436 U/μg protein. One unit will hydrolyze one pmole Z-Leu-Arg-AMC substrate (Prod. No. BML-P229, 25 μM) per minute at 25°C, in 25mM NaOAc pH 5.5, 100mM NaCl, 1.0mM DTT.
Product Name Alternative
Cathepsin L2
Certification
RUO
UniProt
O60911
Source
Produced in insect cells. Recombinant glycosylated procathepsin V cloned from human cDNA and purified as full-length proenzyme. Produced in a baculovirus expression system.
Stability
Avoid extended periods unfrozen. This enzyme is stable for 6 months when stored as received under the above conditions.
Purity
≥90%
Molecular Weight
~37kDa (SDS-PAGE)
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Notes
The proenzyme can be activated as in Adachi et al. (Reference year 2008) : Dilute proenzyme into 200mM NaAcetate, pH 6.0, with 0.05% SDS, 2.5mM DTT, and incubate at 37°C for 5-30 minutes. Alternatively, pre-incubate proenzyme 5-30 minutes in assay buffer (25mM NaOAc pH 5.5, 100mM sodium chloride, 1.0mM DTT), then add substrate to begin assay. Incubation times must be determined empirically; activation is dependent on factors such as buffer, temperature, and enzyme concentration, and cathepsin V will autodegrade once activated.
Formulation
Liquid. In 25mM TRIS-HCl, pH 8.0, containing 100mM sodium chloride, 0.05% Tween-20 and 10% glycerol.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates and screen for inhibitors.
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