Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4) (HRP conjugate)
Selected Variant
BML-PW0605-0025
Size: 25 µg
Product Specifications
Certification
RUO
UniProt
P0CG47 (UBB), P0CG48 (UBC)
Host
Mouse
Species Reactivity
Species independent
Immunogen
Synthetic peptide containing the ubiquitin K63-isopeptide motif.
Clone
HWA4C4
Source
Purified from hybridoma tissue culture supernatant.
Applications
WB
Purification
Protein G-affinity purified.
Dilution
Western Blot (1:1,000) Specificity for K63-isopeptide-linked polyubiquitin conjugates is temperature sensitive. Western blot membranes MUST NOT be autoclaved and excessive heat should avoided.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes K 63 -linked polyubiquitin in wide range of species.
Formulation
Liquid. In 1X PBS, pH 7.2.
Applications Notes
K 63 -linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blotting compared to equivalent experiments using general ubiquitin antibodies, despite the enhanced detection capability of the HWA4C4 HRP conjugated antibody.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG2a
Cross Reactivity Details
Does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species or free ubiquitin.
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