Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4)

Immunocytochemistry (1:100) Immunohistochemistry (1:100) Western Blot (1:500 – 1:1,000) Specificity for K63 of mAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) is temperature sensitive. Western blot membranes must not be autoclaved. Excessive heat should be avoided in all applications. K63-linked polyubiquitin modified proteins comprise only a proportion of total cellular polyubiquitinylated species. Increased sample loading may therefore be required for analysis by Western blot. Do not use milk as blocking solution. Use 1% BSA in an appropriate buffer instead.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.