PO4-AM3

PO4-AM3 is rather labile in aqueous solution. Therefore, aqueous solutions should be freshly prepared immediately before use. Stock solutions in anhydrous DMSO or DMF should be relatively stable when stored frozen at -20°C to -70°C.

Due to its rather high lipophilicity, the solubility of PO4-AM3 in water or buffers is limited. We suggest to use a small amount of anhydrous organic solvent such as anhydrous DMSO or DMF for preparation of 1-100mM stock solutions, and to dilute with water or buffer down to the concentrations required. In some cases, especially at high concentrations (~ 1mM), Pluronic® F-127 (Molecular Probes) can be useful to facilitate solubilization in water or physiological media. Please be sure to check for DMSO/DMF tolerance in your system. Since PO4-AM3 is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise, serum esterases may strongly reduce the cell-loading efficacy. Rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.