PLAQPROTM Lp-PLA2Assay

Store all components, with the exception of Reagent 1, at -80°C until the kit’s expiration date. Reagent 1 may be stored at or below -20°C. Once thawed, the components can be stored at 2-8°C for up to 4 weeks.

The PLAQPRO TM Lp-PLA 2 Assay is a colorimetric readout enzyme activity assay. During the test, Lp-PLA 2 in serum samples hydrolyze the S N 2 position of the substrate, 1-myristoyl-2- (4-nitrophenylsuccinyl) phosphatidylcholine. The resulting colored reaction product, 4-nitrophenol, is measured spectrophotometrically and the rate of formation of 4-nitrophenol is determined over successive measurements. The assay kit includes five Lp-PLA 2 calibrator standards for use in generating a standard curve fit of change in absorbance versus Lp-PLA 2 activity level in nmol/min/mL. The activity of Lp-PLA 2 in serum samples is interpolated from the calibration curve.