Phosphotyrosine monoclonal antibody (16F4)
Product Specifications
Certification
RUO
Host
Mouse
Species Reactivity
Species independent
Immunogen
Phosphotyrosine containing peptides conjugated to KLH.
Clone
16F4
Applications
ELISA, IP, WB
Purification
Thiophilic adsorption and size exclusion chromatography purified.
Positive Control
Included. (Prod. No. 850-051-KI02)
Stability
Stable at -80°C up to 1 year, at 4°C up to 3 months.
Dilution
Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Epitope: ...DIpYAE...Recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating hydrophobic amino acids directly neighbouring the phosphotyrosine.
Formulation
Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
Frequently Asked Questions
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