Phosphoserine monoclonal antibody (4A3)
Product Specifications
Certification
RUO
Host
Mouse
Species Reactivity
Species independent
Immunogen
Phosphoserine containing peptides conjugated to KLH.
Clone
4A3
Applications
ELISA, IP, WB
Purification
Thiophilic adsorption and size exclusion chromatography purified.
Positive Control
Included. (Prod. No. 840-012-1)
Stability
Stable at -80°C up to 1 year, at 4°C up to 1 week.
Dilution
Western Blot (1μg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20. Do not use milk/casein for blocking and dilution! Replace by BSA!) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature) .
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with phosphoserine surrounded by positive or neutral aa (e.g. substrates of PKA, PKB, PKC, PKG etc.) . Please note that phosphoserine detection by MAbs always depends on the surrounding aa sequence.
Formulation
Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgM
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