P-glycoprotein monoclonal antibody (C219)

Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.Tissue: Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretory epithelium) are recommended.

Immunohistochemistry (paraffin sections) :- For optimal staining, the sections should be pretreated with an antigen unmasking solution.- The antibody may be diluted to ≥1:25 for biotin based detection systems.- For optimal staining, the primary antibody should be incubated 60 minutes at room temperature.Western Blot (1:50-1:100) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein.

Flow Cytometry: cell permeabilization required. Detects bands of ~170kDa (MDR1 P-glycoprotein) and of ~140kDa (MDR3 P-glycoprotein) by Western blot. Cited applications include ELISA, IF, FC, and WB