P-glycoprotein (human) monoclonal antibody (MRK16)

Product Specifications

Product Name Alternative

ABCB1, CD243, P-glycoprotein 1

Certification

RUO

UniProt

P08183

Host

Mouse

Species Reactivity

Human

Immunogen

Adriamycin-resistant human myelogenous leukemia K-562/ADM cells.

Clone

MRK16

Applications

Flow Cytometry, FUNC, IHC (FS), IP

Purification

Protein A affinity purified.

Dilution

Flow Cytometry: For 5x105 pelleted cells use 50μl of 10-100μg/ml.Immunohistochemistry (frozen sections) : 0.1-10μg/ml. Use mild fixation treatment such as 4% paraformaldehyde. Do not use acetone, paraffin, methanol or ethanol as a fixative. Iodine labelling can also be performed on MRK16.Immunoprecipitation: 25-50μg/mlSuggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Specificity

Recognizes an external epitope of human P-glycoprotein. Also recognizes P-glycoprotein of human cortical adenomas.

Formulation

Liquid. In PBS containing 1% BSA and 0.1% sodium azide.

Applications Notes

Functional Application: Inhibits P-glycoprotein function. Not recommended for Western blot (epitope recognized by this antibody may be lost in the presence of protein denaturing agents such as SDS) .