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O-GlcNAc monoclonal antibody (CTD110.6) (purified)

Product Specifications

Product Name Alternative

β-O-linked N-acetylglucosamine

Certification

RUO

Host

Mouse

Species Reactivity

Species independent

Immunogen

Synthetic peptide containing serine-O-GlcNAc.

Clone

CTD110.6

Applications

IF, IHC (PS)

Purification

Purified by affinity chromatography.

Dilution

For immunofluorescence microscopy, a concentration range of 2.5 – 10 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 – 10 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Shipping Conditions

Blue Ice

Storage Conditions

+4°C

Specificity

Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc.

Formulation

Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.

Applications Notes

Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.

Warranty

This antibody is covered by our Worry-Free Guarantee .

Isotype

IgM

Cross Reactivity Details

Does not cross-react with peptide determinants or other closely-related carbohydrate antigens.

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