O-GlcNAc monoclonal antibody (CTD110.6) (purified)
Product Specifications
Product Name Alternative
β-O-linked N-acetylglucosamine
Certification
RUO
Host
Mouse
Species Reactivity
Species independent
Immunogen
Synthetic peptide containing serine-O-GlcNAc.
Clone
CTD110.6
Applications
IF, IHC (PS)
Purification
Purified by affinity chromatography.
Dilution
For immunofluorescence microscopy, a concentration range of 2.5 – 10 μg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 – 10 μg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Conditions
Blue Ice
Storage Conditions
+4°C
Specificity
Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc.
Formulation
Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Applications Notes
Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgM
Cross Reactivity Details
Does not cross-react with peptide determinants or other closely-related carbohydrate antigens.
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