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NUCLEOLAR-ID® Green detection kit

Product Specifications

Certification

RUO

Applications

Fluorescence microscopy, Fluorescent detection

Stability

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Notes

The NUCLEOLAR-ID ® Green detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Applications Notes

Specifically designed for visualizing microscopically nucleoli in living cells.

Quality Control

A sample from each lot of NUCLEOLAR-ID ® Green Detection Kit is used to stain HeLa cells using the procedures described in the user manual. Cells stained with NUCLEOLAR-ID ® Green Detection Reagent show maximal fluorescence signal within the nucleoli, with only faint fluorescence throughout the nucleus and cytoplasm. Cells induced by actinomycin D show reduction of nucleolar signal.

Contents

NUCLEOLAR-ID ® Green Detection Reagent , 50 μL Actinomycin D Control , 125 μg 10X Assay Buffer , 15 mL

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