NUCLEAR-ID®Red cell cycle kit (GFP-CERTIFIED®)

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

The GFP-CERTIFIED ® NUCLEAR-ID ® Red Cell Cycle Analysis Kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

Absorption peak of NUCLEAR-ID ® Red dye: λ max = 566 ± 4 nm % purity of NUCLEAR-ID ® Red dye by HPLC: ≥93% A sample from each lot of GFP-CERTIFIED ® NUCLEAR-ID ® Red Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. Cells with Nocodazole gave %G 2 value of >60%. Untreated cells gave the following results: (a) G 0 /G 1 peak CV < 15%; (b) %G 1 > 35%; (c) %G 2 < 15%; and (d) G 2 /G 1 ratio > 1.8.