NUCLEAR-ID® Green chromatin condensation detection kit
Product Specifications
Certification
RUO
Applications
Flow Cytometry, Fluorescence microscopy, Fluorescent detection
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Applications Notes
This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.
Quality Control
Absorption peak of NUCLEAR-ID ® Green dye: λ max = 507 ± 4 nm % purity of NUCLEAR-ID ® Green dye by HPLC: ≥93% A sample from each lot of NUCLEAR-ID ® Green Cell Cycle Analysis Kit is used to analyze Jurkat cells using the procedures described in the user manual. The following results are obtained: (a) Untreated control cells: Viable cells: >85%; Apoptotic cell population: <15% (b) Apoptosis inducer treated cells: Viable cells: >60%; Apoptotic cell population: <30% (c) Mean fluorescence of apoptotic cells / mean fluorescence of viable cells: >40
Contents
NUCLEAR-ID ® Green Cell Cycle Detection Reagent, 100 μl Apoptosis inducer (Staurosporine), 50nmoles 10X Assay Buffer, 30 ml
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