Neutrophil elastase (human), (purified)
Product Specifications
Specifications
20-22 U/mg protein. One unit will hydrolyze 1.0 μmole of MeOSuc-Ala-Ala-Pro-Val-pNA (Prod. No. BML-P213) per minute, at 25°C in 100mM Tris-HCl, pH 7.5 with 500 mM NaCl.
Product Name Alternative
Leukocyte elastase, LE
Certification
RUO
UniProt
P08246
Source
Isolated from human neutrophils. Active enzyme.
Stability
As indicated on product label or CoA when stored as recommended. This enzyme is stable when stored under the above conditions. Procedures such as dilution of the enzyme followed by refreezing could lead to loss of activity.
Purity
≥95% (SDS-PAGE) . Although this is in highly purified form, and the source material tested negative for HbsAg, anti-HBc, anti-HCV, and anti-HIV-1 and -2, handle this product with all due precautions.
Reconstitution
Reconstitute with 50mM sodium acetate, pH 5.5, with 150mM sodium chloride to the original volume, followed by gentle mixing to ensure adequate homogenization.
Shipping Conditions
Dry Ice
Storage Conditions
-20°C
Applications Notes
Useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture.
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