Netrin-1 (mouse), (recombinant)
Product Specifications
Certification
RUO
UniProt
O09118
Source
Produced in HEK 293 cells. The extracellular domain of mouse netrin-1 (aa 27-604) is fused at the C-terminus to a FLAG ® -tag.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Bio Whittaker) .
Purity
≥90% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
Molecular Weight
~80kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse UNC5B.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to UNC5B.
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