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Netrin-1 (human), (recombinant)

Product Specifications

Certification

RUO

UniProt

O95631

Source

Produced in HEK 293 cells. The extracellular domain of human netrin-1 (aa 28-604) is fused at the N-terminus to a FLAG ® -tag.

Purification

Purified by multi-step chromatography.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Purity

≥90% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile distilled water. Resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.

Molecular Weight

~85kDa

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

FLAG is a registered trademark of Sigma-Aldrich Co.

Specificity

Tests positive for binding to human Unc5b by ELISA. Previous lots produced from the same cell line bind to human, mouse and rat receptors UNC5H and DCC.

Formulation

Lyophilized. Contains PBS.

Applications Notes

ELISA: binds to UNC5B at 15-1000 ng/ml.

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