Nerve growth factor 7S (mouse)

Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4 , 79 (1984) .

As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, store for up to one week at +4°C and for 4-6 weeks at -80°C.

Bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Neurotrophic activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and immature sensory nerve cells is 50-100ng/ml medium.

Dilute in sterile water or DMEM to a concentration of at least 10μg/ml and store at -80°C before use. Medium containing NGF cannot be reused due to NGF degradation.