Nerve growth factor 2.5S (mouse), (grade II)
Product Specifications
CAS Number
93928-24-6
Product Name Alternative
NGF 2.5S
Certification
RUO
UniProt
P01139
Source
Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4 , 79 (1984) .
Stability
As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.
Purity
≥95% (HPLC)
Activity
Neurotrophic activity was bioassayed using rat pheochromocytoma cell PC12 cultures over a period of 7-14 days. Activity is in the range of 0.5-50ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium.
Reconstitution
Dilute in sterile water or DMEM to a concentration of at least 10μg/ml and store at -80°C before use.
Molecular Weight
~26kDa; homodimer.
Shipping Conditions
Ambient Temperature
Storage Conditions
-20°C
Formulation
Lyophilized.
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