Nerve growth factor 2.5S (mouse), (grade I)

Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4 , 79 (1984) .

As indicated on product label or CoA when stored as recommended. Stable for 2-3 weeks when stored at room temperature. After reconstitution, stable for up to 1 week when stored at +4°C and for 4-6 weeks at -80°C.

MNGF 2.5S was bioassayed for neurotrophic activity using the rat pheochromocytoma cell PC12 cultures over a period of 7 – 14 days. The neurotrophic activity is in the range of 0.5-50ng/ml. The recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50ng/ml medium.

Reconstitute in sterile water or DMEM at a concentration of at least 10μg/ml and store at -80°C before use. Medium with mNGF cannot be reused due to mNGF degradation.