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Neprilysin (human), (recombinant) (His-tag)

Product Specifications

Product Name Alternative

NEP, Neutral endopeptidase 24.11, Enkephalinase, CALLA, Common acute lymphoblastic leukemia antigen, CD10

Certification

RUO

UniProt

P08473

Source

Produced in Sf9 insect cells. Produced in a baculovirus expression system.

Sequence

NEP from human cDNA, transcript variant 1, aa 53-750 (D 53 – W 750 ; Identical to GeneBank accession NM_000902) is fused at the N-terminus to a His-tag.

Purification

Purified as the glycosylated soluble form.

Stability

Stable on ice for at least 1h. Do not freeze in dilute form.

Purity

≥95% (SDS-PAGE)

Molecular Weight

80.7 kDa (calculated) / ~85 kDa (glycosylated form on SDS-PAGE)

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 50mM TRIS, pH 8.0, containing 300mM sodium chloride and 20% glycerol.

Applications Notes

Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

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