Nephrin (Ig-like domain 5) clonal antibody (Y17-R)

ELISA (1:50,000-1:100,000) Immunohistochemistry (paraffin sections, 1:200) Western Blot (1:5,000; Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100, Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk. Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature) Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Clonal Rabbit Antibody is a pure homogenous fraction of rabbit immunoglobulin (IgG) which corresponds to a unique linear epitope on the target protein. This epitope is designed after a detailed analysis of the antigen structure, regulatory post-translational modifications, protein/protein interaction domain and protein folding. The chosen sequence (peptide) is synthesized and the rabbit is immunized. The clone is then separated from the crude antiserum by “ in vitro cloning technology”. The resulting product is monospecific and characterized by superior specificity, affinity and avidity.