MRP5 (human) monoclonal antibody (M5I-1)

Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.Immunohistochemistry (frozen sections, weak) : Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-rat Ig and a monoclonal rat APAAP complex. Alternatively, after incubation with the primary antibody and washing, slides can be incubated with biotinylated rabbit anti-rat Ig and streptavidin conjugated to horseradish peroxidase.Western Blot: Use 1:20-1:50 dilution and anti-rat-HRP.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.