MRP2 monoclonal antibody (M2III-5)

Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.Immunohistochemistry (frozen sections, paraffin sections) : Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex or on paraffin sections after pretreatment with 0.01 M citric acid, pH 6.0 in distilled water at 100°C for 5 min 3 times. After washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase.Western Blot: Use 1:20-1:50 dilution and anti-mouse-HRP.Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.