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MMP-19 (catalytic domain) (human), (recombinant)

Product Specifications

Specifications

≥ 0.40U/μg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100μM; Prod. No. BML-P125) as substrate.

Product Name Alternative

Matrix metalloproteinase 19, RASI

Certification

RUO

UniProt

Q99542

Source

Produced in E.coli . Active recombinant MMP-19.

Sequence

Recombinant MMP-19 corresponding to aa Leu 99 -Ser 259 (NM_002429), which comprises the catalytic domain of human MMP-19, with a C-terminal purification tag.

Purification

Purified by multi-step chromatography.

Stability

When stored under the recommended conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution followed by refreezing could lead to loss of activity.

Purity

≥95% (SDS-PAGE)

Molecular Weight

19.2 kDa

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 25mM NaBorate, pH 9.3, containing 5mM CaCl 2 , 20μM ZnCl 2 , 0.05% Brij-35 and 20% glycerol.

Applications Notes

Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.

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