MMP-19 (catalytic domain) (human), (recombinant)
Product Specifications
Specifications
≥ 0.40U/μg. One U=100 pmol/min at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100μM; Prod. No. BML-P125) as substrate.
Product Name Alternative
Matrix metalloproteinase 19, RASI
Certification
RUO
UniProt
Q99542
Source
Produced in E.coli . Active recombinant MMP-19.
Sequence
Recombinant MMP-19 corresponding to aa Leu 99 -Ser 259 (NM_002429), which comprises the catalytic domain of human MMP-19, with a C-terminal purification tag.
Purification
Purified by multi-step chromatography.
Stability
When stored under the recommended conditions, this enzyme is stable at the concentration supplied, in its current storage buffer. Procedures such as dilution followed by refreezing could lead to loss of activity.
Purity
≥95% (SDS-PAGE)
Molecular Weight
19.2 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 25mM NaBorate, pH 9.3, containing 5mM CaCl 2 , 20μM ZnCl 2 , 0.05% Brij-35 and 20% glycerol.
Applications Notes
Useful tool to study enzyme kinetics, cleave target substrates, and screen for inhibitors.
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