MMP-10 (catalytic domain) (human), (recombinant)
Product Specifications
Specifications
≥200 pmol/min/μg at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 μM; Prod. No. BML-P125) as substrate.
Product Name Alternative
Matrix metalloproteinase 10, Stromelysin-2
Certification
RUO
UniProt
P09238
Source
Produced in E. coli . Active Matrix Metalloproteinase-10 (MMP-10, stromelysin-2, transin-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-10 (Phe 99 -Glu 271 , NM_2425) with a C-terminal purification tag. This comprises an active form of MMP-10 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected.
Purification
Partially purified by single-step affinity chromatography and gel filtration.
Molecular Weight
19.4 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS, 5mM CaCl 2 , 300mM NaCl, 20μM ZnCl 2 , 0.5% Brij-35, and 30% glycerol.
Applications Notes
Useful tool to study of enzyme kinetics, cleave target substrates, and screen for inhibitors.
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