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MMP-10 (catalytic domain) (human), (recombinant)

Product Specifications

Specifications

≥200 pmol/min/μg at 37°C using the colorimetric thiopeptolide Ac-Pro-Leu-Gly-S-Leu-Leu-Gly-OEt (100 μM; Prod. No. BML-P125) as substrate.

Product Name Alternative

Matrix metalloproteinase 10, Stromelysin-2

Certification

RUO

UniProt

P09238

Source

Produced in E. coli . Active Matrix Metalloproteinase-10 (MMP-10, stromelysin-2, transin-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-10 (Phe 99 -Glu 271 , NM_2425) with a C-terminal purification tag. This comprises an active form of MMP-10 which lacks the C-terminal hemopexin domain. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected.

Purification

Partially purified by single-step affinity chromatography and gel filtration.

Molecular Weight

19.4 kDa

Shipping Conditions

Dry Ice

Storage Conditions

-80°C

Formulation

Liquid. In 50mM TRIS, 5mM CaCl 2 , 300mM NaCl, 20μM ZnCl 2 , 0.5% Brij-35, and 30% glycerol.

Applications Notes

Useful tool to study of enzyme kinetics, cleave target substrates, and screen for inhibitors.

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