Mitochondria/Cytosol fractionation kit
Product Specifications
Certification
RUO
Species Reactivity
Mammalian
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Applications Notes
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
Contents
10ml Mitochondria Extraction Buffer, 20ml 5X Cytosol Extraction Buffer, 100μl DTT (1M), 1 vial Protease Inhibitor Cocktail.
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