MITO-ID®Membrane potential detection kit

The MITO-ID ® Membrane potential detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required. Toxicology Application Note: Use of 3D Cultured Human iPSC-Derived Hepatocytes for Long-Term Hepatotoxicity Studies Monitor Mitochondrial Membrane Potential in Cancer Cell Lines with a Dual-emission Fluorescent Dye

The MITO-ID ® Membrane Potential Detection Kit has been optimized for measurement of mitochondria membrane potential (MMP) and cell viability by conventional fluorescence microscopy and flow cytometry.

In fluorescence microscopy assay, MITO-ID® Membrane Potential dye is a dual-emission probe emitting in the green channel in the cytosol and in the orange channel within the mitochondria. When a perturbing drug such as CCCP is added, the dye exists primarily as green-fluorescent monomers in the cytosol and no longer exhibits orange fluorescence in the mitochondria. Necrosis detection reagent detects dead cells shown in the red channel.