MITO-ID®Green detection kit

The MITO-ID ® Green Detection Kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.

This kit has been specifically designed for use with RFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs) . One important application of MITO-ID ® Green dye is in fluorescence co-localization imaging with red fluorescent protein (RFP) -tagged proteins. This is a powerful approach for determining the targeting of molecules to intracellular compartments, and for screening of associations and interactions between these molecules. Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with fluorescent probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as coumarin, phycoerythrin or cyanine-5. A nuclear counterstain (Hoechst 33342) is provided to highlight this organelle as well.

A sample from each lot of MITO-ID ® Green Detection Kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the MITO-ID ® Green dye is evident.