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MEGACD40L®Protein (soluble) (mouse), (recombinant)

Product Specifications

Product Name Alternative

CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF5:ACRP30headless, gp39:ACRP30headless

Certification

RUO

UniProt

P27548 (CD40L), Q60994 (ACRP30)

Source

Produced in CHO cells. The extracellular domain of mouse CD40L (CD154) (aa 115-260) is fused at the N-terminus to mouse ACRP30 headless (aa 18-111) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of CAPE COD Inc.) .

Purity

≥95% (SDS-PAGE)

Activity

Binds to mouse and human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) .

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Molecular Weight

~35-40kDa

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

FLAG is a registered trademark of Sigma-Aldrich Co.

Formulation

Lyophilized. Contains PBS.

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