MEGACD40L® Protein (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless
Certification
RUO
UniProt
P29965 (human CD40L), Q60994 (mouse Adiponectin)
Source
Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30 headless (aa 18-111) and a FLAG ® -tag.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
≤0.01EU/μg purified protein (LAL test; Cape Cod Associates) .
Purity
≥90% (SDS-PAGE)
Activity
Binds to human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) .
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~38kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Formulation
Lyophilized. Contains PBS.
Applications Notes
Can be used to mediate immune and inflammatory responses, as a protein tool for pre-clinical studies in immunotherapy, to monitor patients undergoing chemotherapy and to mimic autoimmune phenotypes in vivo.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
PKSH031132 100 µg
Recombinant Human CHST11/C4ST-1 Protein (His Tag)
Sign In for Pricing
View Details 
