MEGACD40L® Protein (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
CD40L:ACRP30headless, CD154:ACRP30headless, TNFSF 5:ACRP30headless, gp39:ACRP30headless
Certification
RUO
UniProt
P29965 (human CD40L), Q60994 (mouse Adiponectin)
Source
Produced in CHO cells. The extracellular domain of human CD40L (CD154) (aa 116-261) is fused at the N-terminus to mouse ACRP30 headless (aa 18-111) and a FLAG ® -tag.
Purification
Purified by multi-step chromatography.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
≤0.01EU/μg purified protein (LAL test; Cape Cod Associates) .
Purity
≥90% (SDS-PAGE)
Activity
Binds to human CD40. Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) .
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~38kDa
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
FLAG is a registered trademark of Sigma-Aldrich Co.
Formulation
Lyophilized. Contains PBS.
Applications Notes
Can be used to mediate immune and inflammatory responses, as a protein tool for pre-clinical studies in immunotherapy, to monitor patients undergoing chemotherapy and to mimic autoimmune phenotypes in vivo.
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