Matrix metalloproteinase-2 (MMP-2) fluorometric drug discovery kit, RED

NCBI Reference Sequence: NM_004530 The OMNIMMP ® RED substrate offers key advantages over other MMP substrates. Emission at the red end of the spectrum (576 nm after excitation at 545 nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium. MMP substrate peptides display poor aqueous solubility, often with K m s near their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP K m s for OMNIMMP ® RED substrate are below its solubility limit. In addition to the efficient binding as exhibited by low K m s, OMNIMMP ® RED is avidly cleaved by MMPs, with k cat /K m s in the range of 10 4 -10 6 M -1 sec -1 . The ultra-strong fluorescence of OMNIMMP ® RED allows for substrate concentrations much lower than the K m , a condition generally desirable in inhibitor screening assays.

1 vial MMP-2 enzyme 1 vial substrate (OMNIMMP ® RED) 1 vial 6′-TAMRA calibration standard 1 vial control inhibitor (NNGH) 1 bottle (20 ml) assay buffer 1 black 96-well microplate Instructions