Matrix metalloproteinase (MMP) multipack-1

For MMP-1, MMP-2, MMP-8, and MMP-13: Liquid. 0.1μg/μl in 50mM TRIS, pH 7.5, containing 5mM CaCl 2 , 300mM NaCl, 20μM ZnCl 2 , 30% glycerol, 0.5% Brij-35. For MMP-9: Liquid. In 50mM TRIS, pH 7.5, containing 1mM CaCl 2 , 300mM NaCl, 5μM ZnCl 2 , 0.1% Brij-35 and 15% glycerol.

Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl 2 , 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.

Contains 10μg of each enzyme, provided in a screw-cap microfuge tube. MMP-1 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE180), MMP-2 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE237), MMP-8 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE255), MMP-9 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE360), MMP-13 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE246) .