MALP-2
Product Specifications
Product Name Alternative
Macrophage-activating lipopeptide-2, S-[2,3-bis (Palmityloxy) - (2R) -propyl-cysteinyl-GNNDESNISFKEK]
Certification
RUO
Source
Synthetic.
Stability
As indicated on product label or CoA when stored as recommended. Stable for at least 2 years when stored at -20°C.
Concentration
0.1 mg/mL
Molecular Formula
C 99 H 167 N 19 O 30 S
Molecular Weight
2135.2
Shipping Conditions
Blue Ice
Storage Conditions
-80°C
Notes
MALP-2 sticks avidly to glass or plastic. Since it is active at very low concentrations, i.e. at high dilutions, these low amounts of material tend to become adsorbed to pipette tips and plastic or glass containers. To avoid this, prepare several dilution steps of the MALP-2 stock solution with medium containing 5% autologous serum or buffers with 2% HSA. The presence of 50mM octyl glucoside in the first dilution step is often beneficial and gives rise to higher activity. Avoid small volumes in large vessels. Do not filter.Because of the ester-bound fatty acids, MALP-2 is sensitive to alkali or acid. The medium should be preconditioned in the CO 2 incubator in order to ensure that the pH does not become alkaline. Add cells in preconditioned medium as soon as possible and incubate.
Formulation
Liquid. Sterile solution in PBS containing 2.5% (v/v) 2-propanol, 25mM n-octyl-β-D-glucopyranoside and 1% (wt/v) human serum albumin.
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