LYSO-ID®Red cytotoxicity kit (GFP-CERTIFIED®)
Product Specifications
Certification
RUO
Applications
Fluorescence microscopy, Fluorescent detection
Stability
With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Applications Notes
96-well cell-based assay. Provides a rapid and quantitative approach for determining drug- or toxic agent-induced lysosome and lysosome-like organelle perturbations in live cells.
Quality Control
A sample from each lot of LYSO-ID ® Red cytotoxicity kit (GFP-CERTIFIED ®) is used to stain epithelial cell line, MDCK, using the procedures described in the user manual. The Z’ value, as observed by the fluorescence microplate reader, is > 0.5 for cells treated with 120μM verapamil. The blue nuclear counterstain is at least 70% of the value for the untreated cells.
Contents
10x dual color detection reagent : 2 x 1ml Detection buffer : 20ml Verapamil control : 3μmoles 10x assay buffer : 15ml
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