LSD1 (human), (recombinant)
Product Specifications
Specifications
≥20 U/μg. One U=1 pmol/min at 30°C, 100 μM Histone H3 Dimethyl Lysine-4 Peptide (Prod. No. BML-P256) .
Product Name Alternative
KDM1, Lysine-specific histone demethylase 1, BHC110, AOF2, KIAA0601
Certification
RUO
UniProt
O60341
Source
Produced in E. coli .
Sequence
N-terminal truncation of LSD1 from human cDNA. Sequence is identical to Genbank accession NM015013 (aa 151-852) .
Purification
Purified by multi-step chromatography.
Stability
The enzyme is stable on ice for the time typically required to set up an experiment (30-60 min.), but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
Purity
≥90% (SDS-PAGE)
Molecular Weight
78 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 8.1mM Na 2 HPO 4 , 1.5mM KH 2 PO 4 , pH 7.2, 138mM sodium chloride, 2.7mM KCl and 40% v/v glycerol.
Applications Notes
Useful tool to study LSD1 kinetics and inhibitor sensitivity and also the effect of the enzyme in transcriptional regulation, cell cycle progression, and oncogenesis.
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