LPS from Salmonella abortus equi S-form (TLRGRADE®) (Ready-to-Use) (Biotin)

Smooth (S) -form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered.

Note: As the biotinylation reagent and standard biotinylation protocol favors protein/peptide (contaminants) about 1000-fold over sugar as a target, it is mandatory to use >99.9% pure LPS to exclude the biotinylation of contaminants. These are usually present in many commercial LPS preparations and would lead to misleading observations of the tracking/staining with the reagents. TLRGRADE ® LPS fulfills such high quality requirements and the biotinylation procedure is chosen to preserve TLR4 (Toll-like receptor 4) binding and cellular activation using TLRGRADE ® LPS-Biotin. For the unconjugated LPS, please see ALX-581-009) .