LIGHT (soluble) (human), (recombinant)
Product Specifications
Product Name Alternative
HVEML, TNFSF 14, CD258
Certification
RUO
UniProt
O43557
Source
Produced in HEK 293 cells. The extracellular domain of human LIGHT (aa 89-240) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG ® -tag.
Stability
Stable for at least 6 months after receipt when stored at -20°C.
Concentration
0.1 mg/mL after reconstitution.
Endotoxin
<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .
Purity
≥95% (SDS-PAGE)
Reconstitution
Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
Molecular Weight
~25kDa (SDS-PAGE)
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Historical data shows that the biological activity of LIGHT increases LNCaP (prostate cancer cell) growth. FLAG is a registered trademark of Sigma-Aldrich Co.
Specificity
Binds to human and mouse LTβR and human HVEM and DcR3.
Formulation
Lyophilized. Contains PBS.
Applications Notes
ELISA: binds to HVEM receptor at 1-10 ng/ml.
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