LIGHT (soluble) (human), (recombinant)

Product Specifications

Product Name Alternative

HVEML, TNFSF 14, CD258

Certification

RUO

UniProt

O43557

Source

Produced in HEK 293 cells. The extracellular domain of human LIGHT (aa 89-240) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG ® -tag.

Stability

Stable for at least 6 months after receipt when stored at -20°C.

Concentration

0.1 mg/mL after reconstitution.

Endotoxin

<0.1EU/μg purified protein (LAL test; Associates of Cape Cod) .

Purity

≥95% (SDS-PAGE)

Reconstitution

Reconstitute with 100μl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.

Molecular Weight

~25kDa (SDS-PAGE)

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Notes

Historical data shows that the biological activity of LIGHT increases LNCaP (prostate cancer cell) growth. FLAG is a registered trademark of Sigma-Aldrich Co.