LEXSinduce3 Expression Kit (contains the integrative vector pLEXSY_I-ble3)

LEXSY host T7-TR: kit contains three vials with 1.6 ml each of frozen glycerol stocks of LEXSY host T7-TR. pLEXSY_I-3 expression vector pLEXSY_I-blecherry3 (Cat. No. JBS-EGE-243), 5 μg in 10 mM Tris HCl pH 8.0 pLEXSY_I-3 control plasmid with EGFP gene pLEXSY_I-egfp-ble3 (Cat. No. JBS-EGE-247), 5 μg in 10 mM Tris HCl pH 8.0 Primers for diagnostic PCR and sequencing: ble forward primer A708, for pLEXSY_I-ble3 and pLEXSY_I-blecherry3 Insert sequencing forward primer P1442 Insert sequencing reverse primer A264 5`utr (aprt) reverse primer A1715 odc forward primer A1304 odc reverse primer P1510 LEXSY BHI, Powder for preparation of liquid cultivation medium LEXSY Bleo for pLEXSY_I-ble3 and pLEXSY_I-blecherry3 – 1 ml, ready-to-use 1000x stock solution, 100 mg/ml, filter-sterilized inducer of the T7-TR system: LEXSY Tet (Tetracycline) – 1 ml, ready-to-use 1000x stock solution, 10 mg/ml, filter sterilized Hemin (0.25% porcine Hemin in 50% Triethanolamine) – 2 ml, ready-to-use 500x stock solution, steril-filtered Pen-Strep, ready-to-use 200x stock solution, steril-filtered