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LC3B monoclonal antibody (5F10)

Product Specifications

Product Name Alternative

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

Certification

RUO

UniProt

Q9GZQ8

Host

Mouse

Species Reactivity

Dog,Hamster,Human,Mouse,Rat

Immunogen

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

Clone

5F10

Applications

ICC, WB

Purification

Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.

Positive Control

Included. (Prod. No. ALX-840-038)

Stability

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Dilution

Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 μg/ml) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Specificity

Recognizes both forms of endogenous LC3B.

Formulation

Lyophilized from 1ml PBS containing PEG, sucrose, and 0.09% sodium azide.

Applications Notes

Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.

Warranty

This antibody is covered by our Worry-Free Guarantee .

Isotype

IgG1

Frequently Asked Questions

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