Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

LC3B monoclonal antibody (5F10)

Product Specifications

Product Name Alternative

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

Certification

RUO

UniProt

Q9GZQ8

Host

Mouse

Species Reactivity

Dog,Hamster,Human,Mouse,Rat

Immunogen

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

Clone

5F10

Applications

ICC, WB

Purification

Purified from serum-free cell culture supernatant by subsequent ultrafiltration and size exclusion chromatography.

Positive Control

Included. (Prod. No. ALX-840-038)

Stability

Stable at -80°C up to 1 year, at 4°C up to 3 months.

Dilution

Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 μg/ml) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature) .

Shipping Conditions

Blue Ice

Storage Conditions

-20°C

Specificity

Recognizes both forms of endogenous LC3B.

Formulation

Lyophilized from 1ml PBS containing PEG, sucrose, and 0.09% sodium azide.

Applications Notes

Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.

Warranty

This antibody is covered by our Worry-Free Guarantee .

Isotype

IgG1

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Thioanisole
TRC-T348055-5G 5 g

Thioanisole

Sign In for Pricing
View Details
ERK2 Recombinant Rabbit Monoclonal Antibody [SZ25-01]
ET1603-23 100 µL

ERK2 Recombinant Rabbit Monoclonal Antibody [SZ25-01]

Sign In for Pricing
View Details
Human ERp19 (TXNDC12) activation kit by CRISPRa
GA109527 1 Kit

Human ERp19 (TXNDC12) activation kit by CRISPRa

Sign In for Pricing
View Details
SKA3 (NM_145061) Human Recombinant Protein
TP322931 20 µg

SKA3 (NM_145061) Human Recombinant Protein

Sign In for Pricing
View Details
p53 (TP53) pSer315 Rabbit Polyclonal Antibody
AP12672PU-N 400 µL

p53 (TP53) pSer315 Rabbit Polyclonal Antibody

Sign In for Pricing
View Details
4-(Butylamino)benzoic Acid
TRC-B689445-5G 5 g

4-(Butylamino)benzoic Acid

Sign In for Pricing
View Details