LC3B monoclonal antibody (2G6)

Product Specifications

Product Name Alternative

Microtubule-associated protein 1 light chain 3B, MAP1LC3B

Certification

RUO

UniProt

Q9GZQ8

Host

Mouse

Species Reactivity

Hamster,Human,Monkey,Mouse,Rat

Immunogen

Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.

Clone

2G6

Applications

ICC, WB

Purification

Thiophilic adsorption and size exclusion chromatography purified.

Positive Control

Included. (Prod. No. ALX-840-038)

Stability

Stable at -20°C up to 1 year.

Dilution

Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 μg/ml) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.