LC3B monoclonal antibody (2G6)
Product Specifications
Product Name Alternative
Microtubule-associated protein 1 light chain 3B, MAP1LC3B
Certification
RUO
UniProt
Q9GZQ8
Host
Mouse
Species Reactivity
Hamster,Human,Monkey,Mouse,Rat
Immunogen
Synthetic peptide corresponding to the N-terminus of LC3B conjugated to hemocyanin.
Clone
2G6
Applications
ICC, WB
Purification
Thiophilic adsorption and size exclusion chromatography purified.
Positive Control
Included. (Prod. No. ALX-840-038)
Stability
Stable at -20°C up to 1 year.
Dilution
Immunocytochemistry (paraformaldehyde/methanol fixed cells; 1-10 μg/ml) Western Blot (0.5μg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride) We strongly recommend to use PVDF membranes for immunoblot analysis.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Specificity
Recognizes both forms of endogenous LC3B.
Formulation
Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose.
Applications Notes
Detects a band of ~18kDa (LC3B-I; cytoplasmic form) and a band of ~16kDa (LC3B-II; lipidated form) by Western blot.
Warranty
This antibody is covered by our Worry-Free Guarantee .
Isotype
IgG1
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